Not only did he ignore multiple opportunities to sell ImageJ and make it a commercial program (which would have been hugely lucrative for him) but also for his stroke of genius in incorporating macros and plug-in as an integrative part of the functionality of ImageJ. Over the years ImageJ has worn many hats and it has been used to analyze among other things: autoradiographs, western blot gels, histology, fluorescence, light microscopy, computed axial tomography or positron emission tomography scans and X-raysĮvery time I use the program I cannot help but be amazed at both the scientific integrity and the practical idealism of Wayne Rasband. This program architecture has made of ImageJ a mostly user driven system and its applications are as extensive as they are diverse. NIH Image to ImageJ: 25 years of image analysis Because of macros’ rather basic programming format, general users can create macros with no formal programming experience." "Macros are simple, custom programming scripts that automate tasks inside a large piece of software. Its creator Wayne Rasband, made sure to maintain complexity down, while still achieving versatility through the use of macros, small, easily programmable sets of instructions that can be coded within the whole of ImageJ to add to its functionality. This versatility of ImageJ is achieved in no small part due to its design. It drives the field of biological image processing because it was developed not for one particular use, but it changes according to the needs of each individual user. Western blot data are generally semiquantitative, and analysis often provides relative abundance, not absolute.
#Imagej western blot quantification for free#
(Yes, its a website that gives you a cat GIFs and music, because if you are going to procrastinate you might as well do it right.)īiological image processing is not the exception to this and ImageJ software (and all its derivatives such as FIJI) is available for free for image analysis.Ī great example of a democratic science, ImageJ is an analysis tool that is not only free but also extremely useful and flexible. Protein quantitation in Western blotting. For whatever you need there is a probably a program available in the internet and it is probably free. However as with everything, if there is a need for it, the internet will provide. The algorithms used for these analysis programs here are closed, and hinder collaboration, improvement and progression of the field. There many programs which you can buy that can help you with this problems, these are expensive and often they feature a cookie cutter approach, where the most common applications are covered but they are not easy to customize, often targeting the inexperienced user. Transcribed image text: When you perform a densitometric analysis of a Western Blot using ImageJ what SHOULD YOU NOT do when measuring the same protein on. Thankfully, when my boss is done questioning how I use the word "insurmountable" there are options to effectively tackle problems in image processing.
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